UBAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

UBAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 12385-1-AP

Synonyms

UBAP-1, UBAP 1, UBAP, UAP, Nasopharyngeal carcinoma-associated gene 20 protein

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Validation Data Gallery

• 

  WB analysis of human heart using 12385-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12385-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12385-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12385-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12385-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12385-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12385-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12385-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 12385-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12385-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12385-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12385-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12385-1-AP

  IP result of anti-UBAP1 (IP:12385-1-AP, 4ug; Detection:12385-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12385-1-AP (UBAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12385-1-AP (UBAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 12385-1-AP

  Immunofluorescent analysis of Hela cells, using UBAP1 antibody 12385-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human heart tissue, A431 cells, human lung tissue, human spleen tissue, human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, mouse thymus tissue, Y79 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lung cancer tissue, human heart tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

12385-1-AP targets UBAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBAP1 fusion protein Ag3055
• Full Name: ubiquitin associated protein 1
• Calculated Molecular Weight: 502 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC020950
• Gene Symbol: UBAP1
• Gene ID (NCBI): 51271
• RRID: AB_2211886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.

Publications

Species	Application	Title
human	WB	EMBO J; A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors.; Authors - Paul Rc Imbert
human	WB	Curr Biol; UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.; Authors - Stefani Flavia F
human	WB	J Cell Biol; VPS37A directs ESCRT recruitment for phagophore closure.; Authors - Yoshinori Takahashi
mouse	WB,IF	Cell Rep; ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells; Authors - Danping Lu
human	WB	Cell Rep; The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery.; Authors - Daniel S J Miller
human	WB, IP	Cell Rep; Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration.; Authors - Dmitri Kharitidi

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