UBAC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

UBAC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25122-1-AP

Synonyms

Ubiquitin-associated domain-containing protein 2, UBAC 2, UBA domain-containing protein 2, PSEC0110, Phosphoglycerate dehydrogenase-like protein 1

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Validation Data Gallery

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  WB analysis of MCF-7 using 25122-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25122-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25122-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25122-1-AP (UBAC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 25122-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25122-1-AP (UBAC2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HEK-293 using 25122-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UBAC2 antibody (25122-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:3000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Product Information

25122-1-AP targets UBAC2 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBAC2 fusion protein Ag19142
• Full Name: UBA domain containing 2
• Calculated Molecular Weight: 344 aa, 39 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC121139
• Gene Symbol: UBAC2
• Gene ID (NCBI): 337867
• RRID: AB_2879910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman
human	WB,IHC,IF,RIP	Cell Death Dis; UBAC2 promotes bladder cancer proliferation through BCRC-3/miRNA-182-5p/p27 axis.; Authors - Chaohui Gu ; KD Validated
human	WB	Mol Cell; TMUB1 is an endoplasmic reticulum-resident escortase that promotes the p97-mediated extraction of membrane proteins for degradation; Authors - Linhan Wang
human	WB,IF	EMBO J; ER-phagy restrains inflammatory responses through its receptor UBAC2; Authors - Xing He