UBA3 Monoclonal antibody, PBS Only

UBA3 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C10D8

Cat no : 68067-1-PBS

Synonyms

DKFZp566J164, hUBA3, NEDD8 activating enzyme E1C, UBA3, UBE1C, Ubiquitin activating enzyme 3

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Validation Data Gallery

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  WB analysis using 68067-1-Ig (same clone as 68067-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68067-1-Ig (UBA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68067-1-PBS in a different storage buffer formulation.

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Product Information

68067-1-PBS targets UBA3 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UBA3 fusion protein Ag31051
• Full Name: ubiquitin-like modifier activating enzyme 3
• Calculated Molecular Weight: 463 aa, 52 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC022853
• Gene Symbol: UBA3
• Gene ID (NCBI): 9039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.