TrkB Recombinant antibody, PBS Only

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  WB analysis using 80878-6-RR (same clone as 80878-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80878-6-RR (TrkB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  IP experiment of mouse cerebellum using 80878-6-RR (same clone as 80878-6-PBS)

  IP result of anti-TrkB (IP:80878-6-RR, 4ug; Detection:80878-6-RR 1:4000) with mouse cerebellum tissue lysate 2000 ug. This data was developed using the same antibody clone with 80878-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80878-6-RR

  Biolayer interferometry (BLl) kinetic assays of 80878-6-RR against Human TrkB were performed. The affinity constant is below 1 pM.

TrkB Uni-rAb^TM Recombinant Antibody for WB, IP, Indirect ELISA

Cat No. 80878-6-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

NTRK2, NTRK 2, GP145-TrkB, GP145 TrkB, BDNF/NT-3 growth factors receptor

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Product Information

80878-6-PBS targets TrkB in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TrkB fusion protein Ag3790
• Full Name: neurotrophic tyrosine kinase, receptor, type 2
• Calculated Molecular Weight: 822 aa, 92 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC031835
• Gene Symbol: TrkB
• Gene ID (NCBI): 4915
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q16620
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance by regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes the full-length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).