Transgelin 2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Transgelin 2 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E5G1

Cat no : 60044-1-PBS

Synonyms

SM22 alpha homolog, SM22 beta, SM22beta, TAGLN2, Transgelin 2, Transgelin 2/TAGLN2

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Validation Data Gallery

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  WB analysis of K-562 using 60044-1-Ig (same clone as 60044-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 60044-1-Ig (same clone as 60044-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60044-1-Ig (same clone as 60044-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 60044-1-Ig (same clone as 60044-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 60044-1-Ig (same clone as 60044-1-PBS)

  IP result of anti-Transgelin 2 (IP:60044-1-Ig, 4ug; Detection:60044-1-Ig 1:1000) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60044-1-Ig (same clone as 60044-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60044-1-PBS targets Transgelin 2 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Transgelin 2 fusion protein Ag0337
• Full Name: transgelin 2
• Calculated Molecular Weight: 199 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC002616
• Gene Symbol: TAGLN2
• Gene ID (NCBI): 8407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.