Transferrin Receptor 2/TFR2 Polyclonal antibody

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  WB analysis using 32381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32381-1-AP (TFR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 32381-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32381-1-AP (Transferrin Receptor 2/TFR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Transferrin Receptor 2/TFR2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32381-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TFR2, Transferrin receptor protein 2, transferrin receptor 2, TFRC2, HFE3

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32381-1-AP targets Transferrin Receptor 2/TFR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Transferrin Receptor 2/TFR2 fusion protein Ag37959
• Full Name: transferrin receptor 2
• Calculated Molecular Weight: 801 aa, 89 kDa
• Observed Molecular Weight: 88-97 kDa
• GenBank Accession Number: BC142630
• Gene Symbol: TFR2
• Gene ID (NCBI): 7036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9UP52
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transferrin Receptor 2 (TFR2) is a type II transmembrane glycoprotein that plays a crucial role in iron homeostasis. It is highly homologous to Transferrin Receptor 1 (TFR1) and is primarily expressed in the liver and erythroid precursor cells. TFR2 is involved in the regulation of hepcidin, a hormone that controls iron absorption and distribution in the body. It helps mediate the cellular uptake of iron-bound transferrin, although its affinity for iron is lower than that of TFR1 (PMID: 24658816). It is more involved in sensing iron levels and regulating hepcidin expression rather than direct iron uptake. TFR2 is predominantly expressed in hepatocytes (liver cells) and erythroid precursors (cells that give rise to red blood cells). It is also found in the enterocytes of the small intestine (PMID: 29969719). In the liver, TFR2 acts as a sensor for circulating iron-bound transferrin and helps regulate hepcidin production. Hepcidin, in turn, controls iron absorption from the diet and iron release from macrophages. The molecular weight of TFR2 is 88 kDa, its observed molecular weight is 88-97 kDa.