• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Mouse TNF-alpha Recombinant antibody, PBS Only

TNF-alpha Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240702B7

Cat no : 80258-6-PBS

Synonyms

Tnf, Tnfa, TNF alpha,TNF-a,TNF-alpha,TNFα, TNF alpha, TNF a

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of RAW 264.7 cells using TNF-alpha Recombinant antibody (80258-6-RR)

    WB analysis of RAW 264.7 using 80258-6-RR (same clone as 80258-6-PBS)

    PMA, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80258-6-RR (Tnfa antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80258-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using TNF-alpha Recombinant antibody (80258-6-RR)

    IF Staining of RAW 264.7 using 80258-6-RR (same clone as 80258-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Tnfa antibody (80258-6-RR, Clone: 240702B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80258-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80258-6-RR

    Affinity and Kinetic Characterization of 80258-6-RR

    Biolayer interferometry (BLl) kinetic assays of 80258-6-RR against Mouse Tnfa were performed. The affinity constant is below 1 pM.

Product Information

The immunogen of 80258-6-PBS is TNF-alpha Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name tumor necrosis factor
Observed Molecular Weight25-26 kDa
GenBank Accession NumberNM-013693
Gene Symbol TNF-alpha
Gene ID (NCBI) 21926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Mouse and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the mouse form is glycosylated. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles.