TLR4 Polyclonal antibody

TLR4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 30400-1-AP

Synonyms

CD284, toll like receptor 4

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Validation Data Gallery

• 

  WB analysis using 30400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30400-1-AP (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30400-1-AP (TLR4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30400-1-AP (TLR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: J774A.1 cells, THP-1 cells, NR8383 cells, RAW 264.7 cells, mouse liver tissue, mouse spleen tissue, rat liver tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

30400-1-AP targets TLR4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLR4 fusion protein Ag32998
• Full Name: toll-like receptor 4
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 96 kDa
• GenBank Accession Number: NM_021297
• Gene Symbol: Tlr4
• Gene ID (NCBI): 21898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLR4, also known as CD284, belongs to the Toll-like receptor family. It is a type I transmembrane protein consisting of an extracellular leucine-rich repeat region, a helix transmembrane domain, and an intracellular Toll-IL-1 receptor domain. TLR4 functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. It interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP, and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response.

Publications

Species	Application	Title
mouse,rat	WB	Heliyon; Asprosin promotes vascular inflammation via TLR4-NFκB-mediated NLRP3 inflammasome activation in hypertension; Authors - Rui Ge
mouse	WB	ACS Appl Mater Interfaces; Clinical Grade Fibroin Sutures with Bioactive Gold Clusters Enhance Surgical Wound Healing via Inflammation Modulation; Authors - Fangzhen Tian
mouse	WB	Int J Immunopathol Pharmacol; Corilagin relieves atherosclerosis via the toll-like receptor 4 signaling pathway in vascular smooth muscle cells; Authors - Yujie Wang ; KD Validated