1x10^6 untreated or LPS-treated human PBMCs were surface stained with 0.25 ug Anti-Human Coagulation Factor III/Tissue Factor Rabbit Recombinant Antibody (98104-1-RR, Clone:240784E2) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 untreated or LPS-treated human PBMCs were surface stained with 0.25 ug Anti-Human Coagulation Factor III/Tissue Factor Rabbit Recombinant Antibody (98104-1-RR, Clone:240784E2) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
Affinity and Kinetic Characterization of 98104-1-RR
Biolayer interferometry (BLl) kinetic assays of 98104-1-RR against Human Tissue Factor/CD142 were performed. The affinity constant is 0.834 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98104-1-RR targets Tissue Factor/CD142 in FC applications and shows reactivity with human samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named CD142, coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).
Protocols
Product Specific Protocols
FC protocol for Tissue Factor/CD142 antibody 98104-1-RR
1x10^6 untreated or LPS-treated human PBMCs were surface stained with 0.25 ug Anti-Human Coagulation Factor III/Tissue Factor Rabbit Recombinant Antibody (98104-1-RR, Clone:240784E2) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98104-1-RR
Biolayer interferometry (BLl) kinetic assays of 98104-1-RR against Human Tissue Factor/CD142 were performed. The affinity constant is 0.834 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Tissue Factor/CD142 Recombinant antibody
Catalog Number
98104-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
