Tim23 Monoclonal antibody, PBS Only

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  WB analysis using 67535-1-Ig (same clone as 67535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67535-1-Ig (Tim23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67535-1-Ig (same clone as 67535-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tim23 antibody (67535-1-Ig, Clone: 3H10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

Tim23 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67535-1-PBS

Clone No.3H10F5

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PRO1197, TIM23, TIMM23

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Product Information

67535-1-PBS targets Tim23 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Tim23 fusion protein Ag27069
• Full Name: translocase of inner mitochondrial membrane 23 homolog (yeast)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC062707
• Gene Symbol: Tim23
• Gene ID (NCBI): 10431
• RRID: AB_2882754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O14925
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.