Thrombospondin 1 Monoclonal antibody

Thrombospondin 1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2A3E1

Cat no : 67241-1-Ig

Synonyms

THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1

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Validation Data Gallery

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  WB analysis using 67241-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67241-1-Ig (THBS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 67241-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67241-1-Ig (THBS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67241-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67241-1-Ig (Thrombospondin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, rat spleen tissue, NIH/3T3 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

67241-1-Ig targets Thrombospondin 1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Thrombospondin 1 fusion protein Ag29129
• Full Name: thrombospondin 1
• Calculated Molecular Weight: 129 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: NM_003246
• Gene Symbol: Thrombospondin 1
• Gene ID (NCBI): 7057
• RRID: AB_2882520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.

Publications

Species	Application	Title
rat	WB	Diabetes Metab Syndr Obes; Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats; Authors - Yu Ni
rat	WB,IHC,IF	Phytomedicine; Protective effect of alizarin on vascular endothelial dysfunction via inhibiting the type 2 diabetes-induced synthesis of THBS1 and activating the AMPK signaling pathway; Authors - Mo-Li Zhu