NeutraKine® Thrombin-Coagulation FactorII Monoclonal antibody

NeutraKine® Thrombin-Coagulation FactorII Monoclonal Antibody for Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

2G3G10

Cat no : 69014-1-Ig

Synonyms

Coagulation factor II, F2, EC:3.4.21.5, Activation peptide fragment 2, Activation peptide fragment 1

---

Validation Data Gallery

• 

  Neutralization experiment of NeutraKine® Thrombin-Coagulation FactorII using 69014-1-Ig

  Recombinant human Thrombin (Cat.NO. HZ-3010) catalyzes Spectrozyme PL to form chromophore (monitored absorbance at 405 nm) in a dose dependent manner (left pannel). The activity of Thrombin (400ng/mL HZ-3010) was inihibited by serial dose of anti-Thrombin monoclonal antibody 69014-1-Ig (right pannel). The typically ND50 is 5-20 μg/mL.

---

Neutralization

Product Information

69014-1-Ig targets NeutraKine® Thrombin-Coagulation FactorII in Neutralization, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine Thrombin-Coagulation FactorII protein HZ-3010
• Full Name: coagulation factor II (prothrombin)
• Gene Symbol: F2
• Gene ID (NCBI): 2147
• RRID: AB_2882932
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II, also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.

This antibody can be used to neutralize the bioactivity of Thrombin-Coagulation FactorII.