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Tet1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

ChIP, ChIP-Seq, IHC, WB

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Cat No : 61443,61444,61943 61443

Synonyms

Tet1, 5mC, 5-methylcytosine, 5-hmc, 5-hydroxymethylcytosine, cytosine oxygenase, dna methylation, tet, 5hmc,Tet Methylcytosine Dioxygenase 1, Leukemia-Associated Protein With A CXXC Domain, CXXC6, Ten-Eleven Translocation 1 Gene Protein, LCX, CXXC-Type Zinc Finger Protein 6, CXXC Finger 6, CXXC Zinc Finger 6, Methylcytosine Dioxygenase TET1, Tet Oncogene 1, Ten-Eleven Translocation-1, antibody, polyclonal, western blotting, wb, chip-seq, chromatin, immunoprecipitation, sample

Validation Data Gallery

  • Tet1 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 25 ug of chromatin from a human testicle and 4 ul of TET1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify TET1 binding sites. TET1 ChIP-Seq data shows the expected enrichment of TET1 at CpG islands.

    Tet1 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 25 ug of chromatin from a human testicle and 4 ul of TET1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify TET1 binding sites. TET1 ChIP-Seq data shows the expected enrichment of TET1 at CpG islands.

Product Information

Tested Applications ChIP, ChIP-Seq, IHC, WB

Applications Validated by Active Motif: ChIP: 4 ul per ChIP ChIP-Seq: 4 ul each For TET1, we also offer AbFlex TET1 Recombinant Antibody (rAb). For details, see Catalog No. 91171.
Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the C-terminal region of mouse Tet1.
Full Name Tet1 antibody (pAb)
Synonyms Tet1, 5mC, 5-methylcytosine, 5-hmc, 5-hydroxymethylcytosine, cytosine oxygenase, dna methylation, tet, 5hmc,Tet Methylcytosine Dioxygenase 1, Leukemia-Associated Protein With A CXXC Domain, CXXC6, Ten-Eleven Translocation 1 Gene Protein, LCX, CXXC-Type Zinc Finger Protein 6, CXXC Finger 6, CXXC Zinc Finger 6, Methylcytosine Dioxygenase TET1, Tet Oncogene 1, Ten-Eleven Translocation-1, antibody, polyclonal, western blotting, wb, chip-seq, chromatin, immunoprecipitation, sample
Molecular weight 215 kDa
GenBank accession numberNP_081660
RRIDAB_2793639
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Tet1 (Ten-Eleven Translocation-1; methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification.