Talin-1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Talin-1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2E12D10

Cat no : 67992-1-PBS

Synonyms

TLN1, TLN, talin 1, KIAA1027, ILWEQ

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Validation Data Gallery

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  WB analysis using 67992-1-Ig (same clone as 67992-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67992-1-Ig (Talin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67992-1-Ig (same clone as 67992-1-PBS)

  WB result of Talin-1 antibody (67992-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Talin-1 transfected HeLa cells. This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 67992-1-Ig (same clone as 67992-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67992-1-Ig (Talin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67992-1-Ig (same clone as 67992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67992-1-Ig (same clone as 67992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67992-1-Ig (same clone as 67992-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (67992-1-Ig, Clone: 2E12D10 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67992-1-Ig (same clone as 67992-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Talin-1 (67992-1-Ig, Clone:2E12D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

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Product Information

67992-1-PBS targets Talin-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Talin-1 fusion protein Ag5937
• Full Name: talin 1
• Calculated Molecular Weight: 2541 aa, 270 kDa
• Observed Molecular Weight: 270 kDa
• GenBank Accession Number: BC042923
• Gene Symbol: Talin-1
• Gene ID (NCBI): 7094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).