TYROBP/DAP12 Polyclonal antibody

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  WB analysis using 28138-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 28138-1-AP (TYROBP / DAP12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

TYROBP/DAP12 Polyclonal Antibody for WB, ELISA

Cat No. 28138-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

TYROBP, KAR-associated protein, KARAP, KAR associated protein, DNAX-activation protein 12

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Tested Applications

• Positive WB detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28138-1-AP targets TYROBP/DAP12 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TYROBP/DAP12 fusion protein Ag27252
• Full Name: TYRO protein tyrosine kinase binding protein
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 10-12 kDa
• GenBank Accession Number: BC011175
• Gene Symbol: TYROBP
• Gene ID (NCBI): 7305
• RRID: AB_3669646
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43914
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TYROBP, also known as DAP12 or KARAP, is a transmembrane protein consisting of a very small extracellular region, a transmembrane domain containing an aspartic acid residue critical for association with its partner subunits, and an intracellular domain with a single immunoreceptor tyrosine-based activation motif (ITAM) (PMID: 11114420). It is expressed as a disulfide-bonded homodimer on natural killer cells, myeloid cells and a subset of T cells (PMID: 11114420). TYROBP acts as a signaling adaptor that provides signaling function via the Syk and ZAP-70 tyrosine kinase activation pathways (PMID: 15895090). The gene of TYROBP is located on the long arm of chromosome 19 at position 13.1. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Berberine modulates microglial polarization by activating TYROBP in Alzheimer's disease; Authors - Yu Yang