TYK2 Recombinant antibody, PBS Only

TYK2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230176B6

Cat no : 83161-5-PBS

Synonyms

tyrosine kinase 2, JTK1, EC:2.7.10.2, 230176B6

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Validation Data Gallery

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  IF Staining of HepG2 using 83161-5-RR (same clone as 83161-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TYK2 antibody (83161-5-RR, Clone: 230176B6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83161-5-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 83161-5-RR (same clone as 83161-5-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Tyk2 Recombinant Antibody (83161-5-RR, Clone:230176B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83161-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83161-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83161-5-RR against Human TYK2 were performed. The affinity constant is 0.153 nM.

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Product Information

The immunogen of 83161-5-PBS is TYK2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TYK2 fusion protein Ag28457
• Full Name: tyrosine kinase 2
• Calculated Molecular Weight: 1187 aa, 134 kDa
• GenBank Accession Number: BC014243
• Gene Symbol: TYK2
• Gene ID (NCBI): 7297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.