TYK2 Polyclonal antibody

TYK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16412-1-AP

Synonyms

tyrosine kinase 2, JTK1, EC:2.7.10.2

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Validation Data Gallery

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  WB analysis using 16412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16412-1-AP (TYK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16412-1-AP (TYK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16412-1-AP (TYK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 16412-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TYK2 antibody (16412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, RAW 264.7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16412-1-AP targets TYK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TYK2 fusion protein Ag9462
• Full Name: tyrosine kinase 2
• Calculated Molecular Weight: 1187 aa, 134 kDa
• Observed Molecular Weight: 134 kDa
• GenBank Accession Number: BC014243
• Gene Symbol: TYK2
• Gene ID (NCBI): 7297
• RRID: AB_2918047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Biomed Pharmacother; JAK1/JAK2 degraders based on PROTAC for topical treatment of atopic dermatitis; Authors - Junchao Wu
mouse	WB	Dev Cell; Secreted PTEN binds PLXDC2 on macrophages to drive antitumor immunity and tumor suppression; Authors - Cheng Zhang