K-562 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 15289-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
HL-60 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HL-60 using 15289-1-AP
IP result of anti-TXNL1 (IP:15289-1-AP, 4ug; Detection:15289-1-AP 1:300) with HL-60 cells lysate 960ug.
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human thyroid cancer using 15289-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human thyroid cancer using 15289-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of RAW 264.7 using 15289-1-AP
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using 15289-1-AP (TXNL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using 15289-1-AP (TXNL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human heart tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
RAW 264.7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:100-1:400
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
TXNL1(Thioredoxin-like protein 1) is also named as TRP32, TXL, TXNL and belongs to the thioredoxin family. It might be involved in the cellular response to sugar starvation stress. The predicted 289-amino acid, 32.3-kD protein has 2 distinct domains: an N-terminal domain, which is 43% identical to human TXN, and a C-terminal domain, which showed no homology to other proteins in the sequence databases(PMID:9473519).TXNL1 functions as an effector of oxidants or redox sensor that couples oxidative stress to endocytosis, by converting redox changes into a specific GDI:Rab5-mediated endocytic response(PMID:17987124).
K-562 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 15289-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human heart using 15289-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 15289-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human thyroid cancer using 15289-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human thyroid cancer using 15289-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 40x lens).
IP Figures
IP experiment of HL-60 using 15289-1-AP
IP result of anti-TXNL1 (IP:15289-1-AP, 4ug; Detection:15289-1-AP 1:300) with HL-60 cells lysate 960ug.
IF/ICC Figures
IF Staining of RAW 264.7 using 15289-1-AP
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using 15289-1-AP (TXNL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
TXNL1 Polyclonal antibody
Catalog Number
15289-1-AP
Citations
3
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:100-1:400 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
