TXNIP Polyclonal antibody

TXNIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27429-1-AP

Synonyms

EST01027, HHCPA78, THIF, Thioredoxin binding protein 2, TXNIP, VDUP1

---

Validation Data Gallery

• 

  WB analysis using 27429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27429-1-AP (TXNIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse colon using 27429-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27429-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27429-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27429-1-AP (TXNIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 27429-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TXNIP antibody (27429-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, RAW 264.7 cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27429-1-AP targets TXNIP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TXNIP fusion protein Ag26579
• Full Name: thioredoxin interacting protein
• Calculated Molecular Weight: 391 aa, 44 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC093702
• Gene Symbol: TXNIP
• Gene ID (NCBI): 10628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNIP, also known as VDUP-1 or TBP-2, belongs to alpha-arrestin protein family and is perhaps the only family member known to bind thioredoxin (TRX). TXNIP was induced by Vitamin D3, but not induced by another monocyte or macrophage differentiation inducer: phorbol 12-myristate 13-acetate (PMA). TXNIP bound catalytic active center of thioredoxin (TRX), which protected cells against oxidative stress. TXNIP was found to be a negative regulator of thioredoxin activity and inducer of the intracellular level of reactive oxygen species (ROS). TXNIP plays an important role in a wide variety of biological functions, such as the regulation of cell death, growth, differentiation, and energy metabolism.