TXNDC9 Monoclonal antibody

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• KD/KO Validated

TXNDC9 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2A10G3

Cat no : 68335-1-Ig

Synonyms

Thioredoxin domain-containing protein 9, Protein 1-4, Protein 1 4, PHLP3, ATP-binding protein associated with cell differentiation

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Validation Data Gallery

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  WB analysis using 68335-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68335-1-Ig (TXNDC9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HEK-293 using 68335-1-Ig

  WB result of TXNDC9 antibody (68335-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC9 transfected HEK-293 cells.

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  FC experiment of HEK-293 using 68335-1-Ig

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug TXNDC9 Monoclonal antibody (68335-1-Ig, Clone:2A10G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: U2OS cells, HEK-293 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68335-1-Ig targets TXNDC9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TXNDC9 fusion protein Ag33259
• Full Name: thioredoxin domain containing 9
• Calculated Molecular Weight: 226 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC005968
• Gene Symbol: TXNDC9
• Gene ID (NCBI): 10190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNDC9, also known as APACD, has a negative effect on protein folding by significantly reducing the activity of chaperone protein TCP1 complex ATPase activity. Including actin or tubulin.