TXNDC5 Monoclonal antibody

TXNDC5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1A12C3

Cat no : 68609-1-Ig

Synonyms

Hcc 2, ERp46, ER protein 46, EndoPDI, EC:1.8.4.-

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Validation Data Gallery

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  WB analysis using 68609-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68609-1-Ig (TXNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

• Positive WB detected in: A549 cells, COLO 320 cells, HepG2 cells, LNCaP cells, Jurkat cells, K-562 cells, TF-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68609-1-Ig targets TXNDC5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TXNDC5 fusion protein Ag13965
• Full Name: thioredoxin domain containing 5 (endoplasmic reticulum)
• Calculated Molecular Weight: 432 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC001199
• Gene Symbol: TXNDC5
• Gene ID (NCBI): 81567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; TXNDC5 Plays a Crucial Role in Regulating Endoplasmic Reticulum Activity through Different ER Stress Signaling Pathways in Hepatic Cells; Authors - Seyed Hesamoddin Bidooki