TXNDC5 Polyclonal antibody

• Featured Product
• KD/KO Validated

TXNDC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 19834-1-AP

Synonyms

EndoPDI, ER protein 46, ERP46, Hcc 2, PDIA15, Thioredoxin like protein p46, TLP46, TXNDC5, UNQ364

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Validation Data Gallery

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  WB analysis using 19834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 19834-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 19834-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19834-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19834-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 19834-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNDC5 antibody (19834-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human skeletal muscle tissue, COLO 320 cells, mouse colon tissue, human placenta tissue
• Positive IHC detected in: human stomach cancer tissue, human stomach tissue, human colon cancer tissue, human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 19 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below

Product Information

19834-1-AP targets TXNDC5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TXNDC5 fusion protein Ag13808
• Full Name: thioredoxin domain containing 5 (endoplasmic reticulum)
• Calculated Molecular Weight: 432 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC001199
• Gene Symbol: TXNDC5
• Gene ID (NCBI): 81567
• RRID: AB_10644285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF,IP	Sci Adv; Targeting mechanosensitive endothelial TXNDC5 to stabilize eNOS and reduce atherosclerosis in vivo.; Authors - Chih-Fan Yeh ; KD Validated
human,mouse	WB,IHC,IF	Nat Commun; Fibroblast-enriched endoplasmic reticulum protein TXNDC5 promotes pulmonary fibrosis by augmenting TGFβ signaling through TGFBR1 stabilization.; Authors - Tzu-Han Lee ; KO Validated
mouse	IHC,IF,WB,CoIP,FC	J Clin Invest; Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGFβ signaling in kidney fibroblasts.; Authors - Yen-Ting Chen ; KD Validated
mouse	WB,CoIP	Int J Mol Sci; Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell Line; Authors - Seyed Hesamoddin Bidooki
mouse	WB	Antioxidants (Basel); Squalene Loaded Nanoparticles Effectively Protect Hepatic AML12 Cell Lines against Oxidative and Endoplasmic Reticulum Stress in a TXNDC5-Dependent Way.; Authors - Seyed Hesamoddin Bidooki
mouse	WB	Antioxidants (Basel); Counteracting Cisplatin-Induced Testicular Damages by Natural Polyphenol Constituent Honokiol.; Authors - Tse-En Wang