Thioredoxin Monoclonal antibody

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• KD/KO Validated

Thioredoxin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F3C1

Cat no : 66475-1-Ig

Synonyms

ADF, ATL derived factor, SASP, thioredoxin, TRDX, TRX, TRX1, TXN

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Validation Data Gallery

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  WB analysis using 66475-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66475-1-Ig (TXN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 66475-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66475-1-Ig (Thioredoxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66475-1-Ig

  WB result of Thioredoxin antibody (66475-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thioredoxin transfected HeLa cells.

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  IHC staining of human ovary tumor using 66475-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66475-1-Ig (Thioredoxin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66475-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66475-1-Ig (Thioredoxin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66475-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66475-1-Ig (Thioredoxin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66475-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Thioredoxin antibody (66475-1-Ig, Clone: 1F3C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, MCF-7 cells, HeLa cells, HepG2 cells, A549 cells, Jurkat cells, HEK-293 cells, K-562 cells
• Positive IHC detected in: human ovary tumor tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66475-1-Ig targets Thioredoxin in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Thioredoxin fusion protein Ag6355
• Full Name: thioredoxin
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC003377
• Gene Symbol: TXN
• Gene ID (NCBI): 7295
• RRID: AB_2881841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.