Thioredoxin Polyclonal antibody

• Featured Product
• KD/KO Validated

Thioredoxin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

78

Reviews (1)

Cat no : 14999-1-AP

Synonyms

TXN, TRDX, THIO, Surface-associated sulphydryl protein, Hom s Trx

---

Validation Data Gallery

• 

  WB analysis using 14999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14999-1-AP (Thioredoxin antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14999-1-AP (Thioredoxin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14999-1-AP

  WB result of Thioredoxin antibody (14999-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thioredoxin transfected HeLa cells.

• 

  IP experiment of HeLa using 14999-1-AP

  IP result of anti-Thioredoxin (IP:14999-1-AP, 4ug; Detection:14999-1-AP 1:6000) with HeLa cells lysate 1520 ug.

• 

  IHC staining of human liver cancer using 14999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14999-1-AP (Thioredoxin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 14999-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14999-1-AP (Thioredoxin antibody) at dilution of 1:900 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14999-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14999-1-AP (Thioredoxin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 14999-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Thioredoxin (14999-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K562 cells, MCF-7 cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:9000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 70 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• IP: See 5 publications below
• ELISA: See 1 publications below
• CoIP: See 3 publications below

Product Information

14999-1-AP targets Thioredoxin in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Thioredoxin fusion protein Ag6989
• Full Name: thioredoxin
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC003377
• Gene Symbol: TXN
• Gene ID (NCBI): 7295
• RRID: AB_2272597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Publications

Species	Application	Title
mouse	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
mouse	WB	Acta Pharm Sin B; Fluorine-thiol displacement probes for acetaminophen's hepatotoxicity; Authors - Benjamin L Prather
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
		Nano Converg; Detection of thioredoxin-1 using ultra-sensitive ELISA with enzyme-encapsulated human serum albumin nanoparticle.; Authors - Myeong-Jun Lee
human	WB	Cell Rep; CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons; Authors - Carissa L Sirois
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Amandine (Verified Customer) (02-07-2024)

20ug of protein, denatured or not, PVDF membrane, fast transfert, primary antibody 1/5000 1h30 RT, secondary antibody 1/5000 2h RT. Chemiluminescence revelation 10sec

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: ImCC

Thioredoxin Antibody Western Blot validation (1/5000 dilution) in ImCC (Cat no:14999-1-AP)

Related Products

PRDX1 Polyclonal antibody

15816-1-AP

peroxiredoxin 2 Polyclonal antibody

10545-2-AP

peroxiredoxin 2 Polyclonal antibody

10545-2-AP

TXNRD1 Polyclonal antibody

11117-1-AP

P53 Polyclonal antibody

21891-1-AP

NFKB1,p105,p50 Polyclonal antibody

14220-1-AP