Alpha Taxilin Polyclonal antibody

• Featured Product
• KD/KO Validated

Alpha Taxilin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27558-1-AP

Synonyms

TXLNA, TXLN, taxilin alpha, RP4 622L5.4, IL14

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Validation Data Gallery

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  WB analysis using 27558-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 27558-1-AP (TXLNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27558-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27558-1-AP (Alpha Taxilin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

27558-1-AP targets Alpha Taxilin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Taxilin fusion protein Ag23001
• Full Name: taxilin alpha
• Calculated Molecular Weight: 546 aa, 62 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC103823
• Gene Symbol: Alpha Taxilin
• Gene ID (NCBI): 200081
• RRID: AB_2880907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-taxilin is a member of the taxilin family which consist of at least three members: alpha-, beta- and gamma-taxilins. Alpha-taxilin was identified as a novel binding partner of the syntaxin family, which is involved in intracellular vesicle traffic. Alpha-taxilin is ubiquitously expressed. It plays an essential role for release of HBV-DNA containing particles. Alpha-taxilin encompasses 546 aa and has a calculated molecular weight of 62 kDa. It migrates on SDS-PAGE with an apparent molecular weight of 75 kDa. (PMID: 12558796; 23816704)

Publications

Species	Application	Title
human	WB,IF	Elife; α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.; Authors - Dandan Ma ; KD Validated