TWSG1 Polyclonal antibody

TWSG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12327-1-AP

Synonyms

TSG, TWSG1

---

Validation Data Gallery

• 

  WB analysis using 12327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12327-1-AP (TWSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12327-1-AP (TWSG1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 12327-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12327-1-AP (TWSG1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 12327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12327-1-AP (TWSG1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12327-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12327-1-AP (TWSG1 Antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue
• Positive IHC detected in: mouse brain tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12327-1-AP targets TWSG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TWSG1 fusion protein Ag2983
• Full Name: twisted gastrulation homolog 1 (Drosophila)
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC020490
• Gene Symbol: TWSG1
• Gene ID (NCBI): 57045
• RRID: AB_2877845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bone morphogenetic proteins (BMPs) are potent morphogens that are involved in cell fate determination, proliferation, apoptosis and adult tissue homeostasis. Twisted gastrulation (TWSG1) is a secreted BMP binding protein that modulates BMP ligand availability in the extracellular space. Several in vitro studies point toward an important role of TWSG1 in the regulation of the immune system (PMID: 21572028).