TWF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TWF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11732-1-AP

Synonyms

Twinfilin-1, Twinfilin 1

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Validation Data Gallery

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  WB analysis using 11732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11732-1-AP (TWF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11732-1-AP (TWF1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11732-1-AP

  WB result of TWF1 antibody (11732-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TWF1 transfected HeLa cells.

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  IHC staining of human lung cancer using 11732-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11732-1-AP (TWF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11732-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11732-1-AP (TWF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11732-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TWF1 antibody (11732-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, HeLa cells, C2C12 cells, mouse testis tissue, rat testis tissue, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

11732-1-AP targets TWF1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TWF1 fusion protein Ag2332
• Full Name: twinfilin, actin-binding protein, homolog 1 (Drosophila)
• Calculated Molecular Weight: 368 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC022344
• Gene Symbol: TWF1
• Gene ID (NCBI): 5756
• RRID: AB_2241259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Biochem Biophys Res Commun; Twinfilin 1 enhances milk bio-synthesis and proliferation of bovine mammary epithelial cells via the mTOR signaling pathway.; Authors - Lu Li ; KD Validated
human	WB	J Thorac Dis; Overexpression of TWF1 promotes lung adenocarcinoma progression and is associated with poor prognosis in cancer patients through the MMP1 signaling pathway; Authors - Kui Zhai ; KD Validated
human,mouse	WB,IHC,CoIP	FASEB J; TWF1 induces autophagy and accelerates malignant phenotype in lung adenocarcinoma via inhibiting the cAMP signaling pathway; Authors - Yu Wang ; KD Validated
mouse	WB	Sci Signal; The actin-binding protein CAP1 represses MRTF-SRF-dependent gene expression in mouse cerebral cortex; Authors - Sharof Khudayberdiev