TUSC3 Monoclonal antibody

• Featured Product
• KD/KO Validated

TUSC3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D3G12

Cat no : 67382-1-Ig

Synonyms

D8S1992, M33, MRT7, N33, OST3A, Protein N33, tumor suppressor candidate 3, TUSC3

---

Validation Data Gallery

• 

  WB analysis of HeLa using 67382-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67382-1-Ig (TUSC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67382-1-Ig

  WB result of TUSC3 antibody (67382-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TUSC3 transfected hela cells.

• 

  WB analysis of human placenta using 67382-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67382-1-Ig (TUSC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67382-1-Ig targets TUSC3 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TUSC3 fusion protein Ag9197
• Full Name: tumor suppressor candidate 3
• Calculated Molecular Weight: 347 aa, 40 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC010370
• Gene Symbol: TUSC3
• Gene ID (NCBI): 7991
• RRID: AB_2882629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUSC3 (tumor suppressor candidate 3), originally named N33, is a potential tumor supressor gene. Decreased expression of TUSC3 has been found in various cancers, including prostate cancer, pancreas cancer and ovary cancer. TUSC3 also known as OST3A, is identified as a part of the oligosaccharyl-transferase (OST) complex and plays a crucial role in protein N-glycosylation. TUSC3 mutations have been found in families with non-syndromic autosomal recessive mental retardation.