TULP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TULP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (2)

Cat no : 13637-1-AP

Synonyms

TUBL3, Tubby-related protein 3, Tubby-like protein 3

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 13637-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13637-1-AP (TULP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 13637-1-AP

  IP result of anti-TULP3 (IP:13637-1-AP, 4ug; Detection:13637-1-AP 1:800) with SH-SY5Y cells lysate 1600ug.

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  IHC staining of human ovary cancer using 13637-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13637-1-AP (TULP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13637-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13637-1-AP (TULP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13637-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13637-1-AP (TULP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human ovary cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 8 publications below

Product Information

13637-1-AP targets TULP3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TULP3 fusion protein Ag4570
• Full Name: tubby like protein 3
• Calculated Molecular Weight: 442 aa, 50 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC032587
• Gene Symbol: TULP3
• Gene ID (NCBI): 7289
• RRID: AB_2211547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The tubby-like protein 3 (TULP3) has been identified as a member of tubby and tubby-like proteins (TULPs) that plays an important role in maintenance and function of neuronal cells during development and post-differentiation. Tulp3 gene is essential for embryonic development in mice. Recently TULP3 has been reported to bind to the IFT (intraflagellar transport)-A complex and promote trafficking of G protein-coupled receptors into primary cilia, which is implicated in regulating neuronal function.

Publications

Species	Application	Title
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	WB,IF	Elife; Multiple ciliary localization signals control INPP5E ciliary targeting; Authors - Dario Cilleros-Rodriguez
mouse	WB	PLoS Genet; Zfp423 Regulates Sonic Hedgehog Signaling via Primary Cilium Function.; Authors - Chen-Jei Hong ; KD Validated
human	WB	J Neurosci; Comparison of ciliary targeting of two rhodopsin-like GPCRs: role of C-terminal localization sequences in relation to cilium type.; Authors - Abhishek Chadha ; KO Validated
human	WB	J Cell Sci; RABL2 positively controls localization of GPCRs in mammalian primary cilia.; Authors - Izumi Dateyama
mouse	IF	J Biol Chem; Deletion of the phosphatase INPP5E in the murine retina impairs photoreceptor axoneme formation and prevents disc morphogenesis.; Authors - Ali S Sharif

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Evan (Verified Customer) (09-30-2019)

One of the worst antibodies I have ever used. I do not believe this antibody accurately detects Tulp3 by western blotting at all. The amount of non-specific bands at all sizes is so bad this antibody is pretty much only good for a protein stain. I have wasted weeks trying to get this antibody to work to no avail. Avoid this product for western blotting at all costs.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T

FH

Francesc (Verified Customer) (09-19-2018)

We previously described that TULP3 (a PIP2-binding protein) accumulates in INPP5E-KO cilia, which also accumulate PIP2 (Garcia-Gonzalo et al. 2015 Dev Cell 34:400-409). We have successfully confirmed those results using Proteintech's anti-TULP3 antibody (13637-1-AP) in both mouse embryonic fibroblasts (MEFs, see my previous review) and in hTERT-RPE1 cells (here). This TULP3 antibody works beautifully, especially in human cells (it also works well in MEFs, but ciliary signal is less intense and lower dilution is needed). As far as I know, this is the best commercial anti-TULP3 available.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: hTERT-RPE1 cells (human origin)

TULP3 Antibody Immunofluorescence, validation (1/200 dilution) in hTERT-RPE1 cells (human origin) (Cat no:13637-1-AP)