Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||human ovary tumor tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13637-1-AP targets TULP3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TULP3 fusion protein Ag4570|
|Full Name||tubby like protein 3|
|Calculated molecular weight||442 aa, 50 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC032587|
|Gene ID (NCBI)||7289|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The tubby-like protein 3 (TULP3) has been identified as a member of tubby and tubby-like proteins (TULPs) that plays an important role in maintenance and function of neuronal cells during development and post-differentiation. Tulp3 gene is essential for embryonic development in mice. Recently TULP3 has been reported to bind to the IFT (intraflagellar transport)-A complex and promote trafficking of G protein-coupled receptors into primary cilia, which is implicated in regulating neuronal function.
Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.
Multiple ciliary localization signals control INPP5E ciliary targeting
Zfp423 Regulates Sonic Hedgehog Signaling via Primary Cilium Function.
Comparison of ciliary targeting of two rhodopsin-like GPCRs: role of C-terminal localization sequences in relation to cilium type.
J Cell Sci
RABL2 positively controls localization of GPCRs in mammalian primary cilia.
J Biol Chem
Deletion of the phosphatase INPP5E in the murine retina impairs photoreceptor axoneme formation and prevents disc morphogenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Evan (Verified Customer) (09-30-2019)
One of the worst antibodies I have ever used. I do not believe this antibody accurately detects Tulp3 by western blotting at all. The amount of non-specific bands at all sizes is so bad this antibody is pretty much only good for a protein stain. I have wasted weeks trying to get this antibody to work to no avail. Avoid this product for western blotting at all costs.
Francesc (Verified Customer) (09-19-2018)
We previously described that TULP3 (a PIP2-binding protein) accumulates in INPP5E-KO cilia, which also accumulate PIP2 (Garcia-Gonzalo et al. 2015 Dev Cell 34:400-409). We have successfully confirmed those results using Proteintech's anti-TULP3 antibody (13637-1-AP) in both mouse embryonic fibroblasts (MEFs, see my previous review) and in hTERT-RPE1 cells (here). This TULP3 antibody works beautifully, especially in human cells (it also works well in MEFs, but ciliary signal is less intense and lower dilution is needed). As far as I know, this is the best commercial anti-TULP3 available.