TUFM Polyclonal antibody

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  WB analysis using 26730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26730-1-AP (TUFM antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 26730-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26730-1-AP (TUFM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TUFM antibody (26730-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 26730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TUFM antibody (26730-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 26730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26730-1-AP (TUFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 26730-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUFM (26730-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

TUFM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 26730-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Synonyms

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

26730-1-AP targets TUFM in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TUFM fusion protein Ag24981
• Full Name: Tu translation elongation factor, mitochondrial
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC010041
• Gene Symbol: TUFM
• Gene ID (NCBI): 7284
• RRID: AB_2880616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49411
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Publications

Species	Application	Title
mouse	IF	Nat Cell Biol; Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties; Authors - Dennis K Jeppesen
zebrafish	WB	Nucleic Acids Res; Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.; Authors - Qinghai Zhang
	WB	Nucleic Acids Res; Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.; Authors - Qinghai Zhang
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li
rat	WB	J Hazard Mater; ZLN005 alleviates PBDE-47 induced impairment of mitochondrial translation and neurotoxicity through PGC-1α/ERRα axis; Authors - Zhiyuan Tian
human	WB	Clin Cancer Res; Antitumor Activity of a Mitochondrial-Targeted HSP90 Inhibitor in Gliomas; Authors - Shiyou Wei