TTLL12 Recombinant antibody

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  WB analysis using 84493-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84493-6-RR (TTLL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84493-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84493-6-RR against Human TTLL12 were performed. The affinity constant is 57.3 pM.

TTLL12 Recombinant Antibody for WB, ELISA

Cat No. 84493-6-RR

Clone No.241904F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Tubulin--tyrosine ligase-like protein 12, Inactive tubulin--tyrosine ligase-like protein 12, 241904F4

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Tested Applications

• Positive WB detected in: HEK-293 cells, SKOV-3 cells, PC-3 cells, HeLa cells, K-562 cells, DU 145 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84493-6-RR targets TTLL12 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TTLL12 fusion protein Ag13804
• Full Name: tubulin tyrosine ligase-like family, member 12
• Calculated Molecular Weight: 644 aa, 74 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC001070
• Gene Symbol: TTLL12
• Gene ID (NCBI): 23170
• RRID: AB_3672006
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q14166
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).