TTK Polyclonal antibody, PBS Only

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  WB analysis using 10381-1-AP (same clone as 10381-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 10381-1-AP (same clone as 10381-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10381-1-AP (same clone as 10381-1-PBS)

  WB result of TTK antibody (10381-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTK transfected HEK-293 cells. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IP experiment of DU 145 using 10381-1-AP (same clone as 10381-1-PBS)

  IP result of anti-TTK (IP:10381-1-AP, 4ug; Detection:10381-1-AP 1:600) with DU 145 cells lysate 1280 ug. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10381-1-AP (same clone as 10381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10381-1-AP (TTK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 10381-1-AP (same clone as 10381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 10381-1-AP (same clone as 10381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 10381-1-AP (same clone as 10381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 10381-1-AP (same clone as 10381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 10381-1-AP (same clone as 10381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 10381-1-AP (same clone as 10381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 10381-1-AP (same clone as 10381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 10381-1-AP (same clone as 10381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10381-1-AP (same clone as 10381-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TTK antibody (10381-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 10381-1-AP (same clone as 10381-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TTK (10381-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TTK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 10381-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

MPS1L1, MPS1, Mps 1, hMPS1, ESK

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Product Information

10381-1-PBS targets TTK in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTK fusion protein Ag0405
• Full Name: TTK protein kinase
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 96-97 kDa
• GenBank Accession Number: BC000633
• Gene Symbol: TTK
• Gene ID (NCBI): 7272
• RRID: AB_2211979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P33981
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mps1 family of kinases colocalizes with mitotic checkpoint proteins in kinetochores and/or spindle pole bodies/centrosomes. TTK has been shown to be a cell cycle-regulated kinase displaying maximum activity during M phase(PMID:15618221). Its kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint(PMID:20937696). TTK mRNA is present at relatively high levels in testis and thymus, tissues which contain a large number of proliferating cells, but is not detected in most other benign tissues (PMID:1639825). It has 2 isoforms produced by alternative splicing (PMID:25137017).