transcription termination factor-Specific Monoclonal antibody

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  WB analysis of HeLa using 60235-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60235-1-Ig (transcription termination factor-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

transcription termination factor-Specific Monoclonal Antibody for WB, ELISA

Cat No. 60235-1-Ig

Clone No.7B3H2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

TTF 1, TTF I, TTF1

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Tested Applications

• Positive WB detected in: Hela cell

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60235-1-Ig targets transcription termination factor-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transcription termination factor, RNA polymerase I
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 106 kDa
• GenBank Accession Number: NM_007344
• Gene Symbol: TTF1
• Gene ID (NCBI): 7270
• RRID: AB_2881359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15361
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTF1 is a multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. It plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5, TTF1 recovers DNA-binding activity. The antibody is specific to TTF1.