BBS8 Polyclonal antibody

BBS8 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12505-1-AP

Synonyms

BBS8, TPR repeat protein 8, TTC8

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Validation Data Gallery

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  WB analysis of mouse testis using 12505-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12505-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12505-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12505-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12505-1-AP

  IP result of anti-BBS8 (IP:12505-1-AP, 3ug; Detection:12505-1-AP 1:400) with HeLa cells lysate 2000ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue, human lung tissue, NIH/3T3 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12505-1-AP targets BBS8 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BBS8 fusion protein Ag3186
• Full Name: tetratricopeptide repeat domain 8
• Calculated Molecular Weight: 531 aa, 60 kDa
• Observed Molecular Weight: 50-52 kDa
• GenBank Accession Number: BC026351
• Gene Symbol: BBS8
• Gene ID (NCBI): 123016
• RRID: AB_2211953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS8 (also known as TTC8) is a subunit of BBSome complex which is necessary for ciliary membrane biogenesis. BBS8 localizes specifically to ciliated structures, such as the connecting cilium of the retina and columnar epithelial cells in the lung. Defects in BBS8 cause Bardet-Biedl syndrome type 8 (BBS8), an genetic disorder characterised by retinal degeneration, obesity, polydactyly, cognitive impairment and kidney failure. BBS8 generates two alternatively spliced isoforms. Western blot analysis using this antibody detected a major band around 60 kD in lung tissue.