TTC37 Polyclonal antibody

TTC37 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24594-1-AP

Synonyms

TPR repeat protein 37, Thespin, Superkiller complex protein 3, SKIC3, Ski3

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Validation Data Gallery

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  WB analysis using 24594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24594-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24594-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24594-1-AP

  IP result of anti-TTC37 (IP:24594-1-AP, 4ug; Detection:24594-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human kidney using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 24594-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 24594-1-AP

  Immunofluorescent analysis of HepG2 cells using 24594-1-AP (TTC37 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human kidney tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

24594-1-AP targets TTC37 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTC37 fusion protein Ag18442
• Full Name: tetratricopeptide repeat domain 37
• Calculated Molecular Weight: 1564 aa, 175 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC056893
• Gene Symbol: TTC37
• Gene ID (NCBI): 9652
• RRID: AB_2879628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTC37 (tetratricopeptide repeat protein 37), also named as Thespin, encodes 1,564 amino acid protein that contains twenty tetratricopeptide (TPR) repeats. TPR repeat-containing motifs are found in a variety of proteins and may mediate protein-protein interactions and chaperone activity. Although not expressed in liver, TTC37 is a widely expressed protein with highest levels of expression in vascular tissues, lymph node, pituitary, lung and intestine. TTC37 is a component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C). Defects in TTC37 are associated with Trico-Hepato-Enteric (THE) syndrome.

Publications

Species	Application	Title
human,mouse	WB	J Clin Invest; Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.; Authors - Kun Yang
human	WB	PLoS Genet; A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies.; Authors - Masakazu Kohda