TSSK6 Polyclonal antibody

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  WB analysis using 14081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14081-1-AP (TSSK6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

TSSK6 Polyclonal Antibody for WB, ELISA

Cat No. 14081-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

SSTK, Small serine/threonine kinase, Serine/threonine-protein kinase SSTK, EC:2.7.11.1, CT72

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14081-1-AP targets TSSK6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSSK6 fusion protein Ag5209
• Full Name: testis-specific serine kinase 6
• Calculated Molecular Weight: 30.3 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC014611
• Gene Symbol: TSSK6
• Gene ID (NCBI): 83983
• RRID: AB_3669176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXA6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSSK6 (testis-specific serine kinase 6) is a testis-specific protein kinase that is highly conserved in mammals and essential for spermatogenesis. In spermatozoa, TSSK6 is expressed after meiosis and is localized to the nucleus of spermatocytes and to the posterior part of the head of spermatocytes. TSSK6-deficient spermatozoa exhibit a defective histone-to-fischerin transition (required for chromatin remodeling after meiosis), as well as a reduction in actin polymerization in spermatocytes. The result is striking morphological defects in the spermatozoa, including nuclear malformations, a hairpin shape with the head facing backward, or complete separation of the head. In addition, Tssk6 is frequently aberrantly expressed in colorectal cancer and exhibits oncogenic activity.