TSSC4 Polyclonal antibody

• Featured Product
• KD/KO Validated

TSSC4 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14531-1-AP

Synonyms

U5 small nuclear ribonucleoprotein TSSC4, Tumor-suppressing subchromosomal transferable fragment candidate gene 4 protein, Tumor-suppressing STF cDNA 4 protein, Protein TSSC4

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Validation Data Gallery

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  IHC staining of human lung using 14531-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14531-1-AP (TSSC4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 14531-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14531-1-AP (TSSC4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse lung using 14531-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14531-1-AP (TSSC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14531-1-AP

  Immunofluorescent analysis of Hela cells, using TSSC4 antibody 14531-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive IHC detected in: human lung tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

14531-1-AP targets TSSC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSSC4 fusion protein Ag6030
• Full Name: tumor suppressing subtransferable candidate 4
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 35-45 kDa
• GenBank Accession Number: BC050616
• Gene Symbol: TSSC4
• Gene ID (NCBI): 10078
• RRID: AB_11232415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation.; Authors - Klára Klimešová ; KD Validated