TSPO/PBR Polyclonal antibody, PBS Only

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  WB analysis of HEK-293 using 15444-1-AP (same clone as 15444-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15444-1-AP (TSPO/PBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15444-1-AP (same clone as 15444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15444-1-AP (TSPO/PBR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15444-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15444-1-AP (same clone as 15444-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15444-1-AP (TSPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15444-1-PBS in a different storage buffer formulation.

TSPO/PBR Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 15444-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

15444-1-PBS targets TSPO/PBR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPO/PBR fusion protein Ag7475
• Full Name: translocator protein (18kDa)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC001110
• Gene Symbol: TSPO
• Gene ID (NCBI): 706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30536
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TSPO, also named Peripheral-type benzodiazepine receptor (PBR), encodes the translocator protein, belonging to tryptophan-rich sensory protein family. The protein is mainly localized in the outer mitochondrial membrane and expressed in steroid-synthesizing tissues (PMID:32881658, PMID:1326278). TSPO is involved in the translocation of cholesterol from the outer to inner mitochondrial membrane (PMID:21119734). In response to injury, TSPO has a upregulated expression in the peripheral nervous system (PMID:32423330, PMID:23251719). TSPO is also as a biomarker in a non-invasive procedure including detecting the inflammation in the heart and atherosclerotic plaques (PMID:24402571).