TSPAN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TSPAN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16058-1-AP

Synonyms

9030418M05Rik, NET 1, RP11 322N21.1, Tetraspan NET 1, tetraspanin 1, Tetraspanin TM4 C, TSPAN 1, TSPAN1

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Validation Data Gallery

• 

  WB analysis using 16058-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16058-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16058-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 16058-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 16058-1-AP (TSPAN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 16058-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16058-1-AP (TSPAN1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 16058-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16058-1-AP (TSPAN1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, COLO 320 cells, rat colon tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

16058-1-AP targets TSPAN1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN1 fusion protein Ag8786
• Full Name: tetraspanin 1
• Calculated Molecular Weight: 241 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC013404
• Gene Symbol: TSPAN1
• Gene ID (NCBI): 10103
• RRID: AB_2878210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN1 is a membrane protein of the tetraspanin superfamily, which is characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. Altered TSPAN1 expression has been associated with the progression of some neoplasms including hepatocellular carcinoma, colorectal, gastric, and breast cancers (PMID: 20890423; 19437569; 18822690; 20680643).

Publications

Species	Application	Title
human	WB,IHC	Autophagy; TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.; Authors - Cefan Zhou ; KD Validated
rat	WB,IP,IF	Cell Mol Life Sci; Tetraspanin1 promotes NGF signaling by controlling TrkA receptor proteostasis.; Authors - Facundo Ferrero Restelli ; KD Validated
human,mouse	WB,IF,CoIP	Neurochem Res; 4,5-Dimethoxycanthin-6-one Inhibits Glioblastoma Stem Cell and Tumor Growth by Inhibiting TSPAN1 Interaction with TM4SF1; Authors - Wei Li