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TSH Beta Polyclonal antibody

TSH Beta Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 27288-1-AP

Synonyms

CHNG4, Thyrotropin alfa, Thyrotropin beta chain, Thyrotropin subunit beta, TSH B, TSH BETA, TSHB

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pituitary tissue using TSH Beta Polyclonal antibody (27288-1-AP)

    IHC staining of human pituitary using 27288-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 27288-1-AP (TSH beta antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary tissue using TSH Beta Polyclonal antibody (27288-1-AP)

    IHC staining of human pituitary using 27288-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 27288-1-AP (TSH beta antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27288-1-AP is TSH Beta Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSH Beta fusion protein Ag25532
Full Name thyroid stimulating hormone, beta
Calculated Molecular Weight 138 aa, 16 kDa
GenBank Accession NumberBC069298
Gene Symbol TSH beta
Gene ID (NCBI) 7252
RRIDAB_2880831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TSH Beta antibody 27288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols