TSGA10 Polyclonal antibody

TSGA10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12593-1-AP

Synonyms

CEP4L, testis specific, 10, TSGA10

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Validation Data Gallery

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  WB analysis of rat testis using 12593-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12593-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 12593-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 12593-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12593-1-AP (TSGA10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 12593-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12593-1-AP (TSGA10 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: rat testis tissue, mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

12593-1-AP targets TSGA10 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSGA10 fusion protein Ag3311
• Full Name: testis specific, 10
• Calculated Molecular Weight: 698 aa, 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC028366
• Gene Symbol: TSGA10
• Gene ID (NCBI): 80705
• RRID: AB_2240967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IHC	Front Cell Dev Biol; miR-129-5p Inhibits Bone Formation Through TCF4.; Authors - Chong Yin
human	WB	Front Cell Dev Biol; Pathogenic Variants in ACTRT1 Cause Acephalic Spermatozoa Syndrome.; Authors - Yanwei Sha
human,mouse	WB，IHC	J Cell Mol Med; MACF1 alleviates aging-related osteoporosis via HES1.; Authors - Chong Yin
human	WB,IF	Clin Genet; TSGA10 is a novel candidate gene associated with acephalic spermatozoa.; Authors - Yan-Wei Sha
human	WB	J Assist Reprod Genet; Pathogenesis of acephalic spermatozoa syndrome caused by splicing mutation and de novo deletion in TSGA10.; Authors - Mingfei Xiang
human	WB,IF	Basic Clin Androl; A novel homozygous TSGA10 missense variant causes acephalic spermatozoa syndrome in a Pakistani family; Authors - Khalid Khan