TSC22D1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TSC22D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10214-1-Ig

Synonyms

RP11 269C23.2, Regulatory protein TSC-22, Regulatory protein TSC 22, KIAA1994, HUCEP-2

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Validation Data Gallery

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  WB analysis of human brain using 10214-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10214-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10214-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 10214-1-Ig

  IP result of anti-TSC22D1 (IP:10214-1-Ig, 4ug; Detection:10214-1-Ig 1:500) with rat brain tissue lysate 4000ug.

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  IHC staining of human brain using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of A549 using 10214-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed A549 cells using 10214-1-Ig(TSC22D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, A549 cells, rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

10214-1-Ig targets TSC22D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSC22D1 fusion protein Ag0287
• Full Name: TSC22 domain family, member 1
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC000456
• Gene Symbol: TSC22D1
• Gene ID (NCBI): 8848
• RRID: AB_2272193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSC22 domain family 1, transforming growth factor beta 1 induced transcript 4, (TSC22D1, synonyms: TSC22, TGFB1I4, MGC17597) is a member of a family of leucine zipper containing transcription factors with repressor activity and belongs to the large family of early response genes. TSC-22 regulates cell growth and differentiation, and cell death. Leucine zipper structure of TSC22 inhibits the anchorage independent growth of cancer cells. TSC-22 is also an important downstream component of peroxisome proliferator-activated receptor (PPAR) and TGF-beta signaling during intestinal epithelial cell differentiation. This antibody is a rabbit polyclonal antibody raised against an internal region of human TSC22D1.

Publications

Species	Application	Title
mouse	WB	Cell Death Differ; TSC-22D1 isoforms have opposing roles in mammary epithelial cell survival.; Authors - Huser C A CA
human,mouse	WB,IHC	Clin Cancer Res; Transforming Growth Factor-{beta}-Stimulated Clone-22 Is an Androgen-Regulated Gene That Enhances Apoptosis in Prostate Cancer following Insulin-Like Growth Factor-I Receptor Inhibition.; Authors - Sprenger Cynthia C T CC ; KD Validated
human	WB,IF	Int J Mol Sci; Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry.; Authors - Ryouta Kamimura
mouse	IP,WB	FEBS J; Multiple TSC22D4 iso-/phospho-glycoforms display idiosyncratic subcellular localizations and interacting protein partners.; Authors - Canterini Sonia S
human	WB,IF	Am J Physiol Heart Circ Physiol; Transforming growth factor-beta1 regulation of C-type natriuretic peptide expression in human vascular smooth muscle cells: dependence on TSC22D1.; Authors - Mendon?a Maria C MC
human	WB,IHC	Biochem Biophys Res Commun; Low levels of TSC22 enhance tumorigenesis by inducing cell proliferation in colorectal cancer.; Authors - Yong Zhou