TRPS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRPS1 Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21938-1-AP

Synonyms

GC79, LGCR, TRPS1, Zinc finger protein GC79

---

Validation Data Gallery

• 

  WB analysis using 21938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21938-1-AP

  WB result of TRPS1 antibody (21938-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRPS1 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 21938-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21938-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 21938-1-AP

  IP result of anti-TRPS1 (IP:21938-1-AP, 4ug; Detection:21938-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

• 

  IF Staining of mouse skin using 21938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skin using 21938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse kidney tissue, mouse lung tissue, mouse testis tissue
• Positive IP detected in: mouse lung tissue
• Positive IF-P detected in: mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

21938-1-AP targets TRPS1 in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRPS1 fusion protein Ag16583
• Full Name: trichorhinophalangeal syndrome I
• Calculated Molecular Weight: 1294 aa, 143 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC125020
• Gene Symbol: TRPS1
• Gene ID (NCBI): 7227
• RRID: AB_11232233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains

Publications

Species	Application	Title
human		Biochim Biophys Acta Mol Basis Dis; UVB-induced TRPS1 regulates MITF transcription activity to promote skin pigmentation; Authors - Yushan Zhang
mouse	IF	Acta Biomater; Shape-Fixing Hydrogel Promotes Scarless Healing of Wounds Under Tension; Authors - Danlan Fu
human	IF	Clin Transl Oncol; Endoplasmic reticulum stress regulates apoptosis and chemotherapeutic via enhancing TNFRSF10B recycling to the cell membrane in triple-negative breast cancer; Authors - Dapeng Zhao