TRPM8 Polyclonal antibody

TRPM8 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12813-1-AP

Synonyms

Trp-p8, TRPP8, Trp p8, LTrpC-6, LTrpC6

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Validation Data Gallery

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  IHC staining of human liver cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12813-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12813-1-AP (TRPM8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 12813-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 12813-1-AP (TRPM8 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive IHC detected in: human liver cancer tissue, human prostate cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

12813-1-AP targets TRPM8 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRPM8 fusion protein Ag3483
• Full Name: transient receptor potential cation channel, subfamily M, member 8
• Calculated Molecular Weight: 1104 aa, 128 kDa
• GenBank Accession Number: BC001135
• Gene Symbol: TRPM8
• Gene ID (NCBI): 79054
• RRID: AB_2877883
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPM8, also known as CMR1 (cold-menthol receptor-1), is a nonselective cation channel activated by cold and the cooling substances menthol and icilin (PMID: 11893340; 11882888). It serves as a neuronal sensor of cold temperatures. Functioning TRPM8 channels are required for behavioral responses to innocuous cool, noxious cold, injury-evoked cold hypersensitivity, and cooling-mediated analgesia (PMID: 17538622; 21438154; 16920620; 21984952). Additionally, TRPM8 has also been reported to be involved in thermoregulation and the regulation of basal tear flow (PMID: 21407809; 21076394). TRPM8 is expressed extensively in sensory nerves, human prostate and overexpressed in a variety of cancers including prostate, breast, lung, colon and skin melanomas (PMID: 11325849; 20816009).

Publications

Species	Application	Title
mouse	IF	Biomaterials; Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.; Authors - Xiao-Tao He
mouse	IF	Inflammation; Cold Stimuli Facilitate Inflammatory Responses Through Transient Receptor Potential Melastatin 8 (TRPM8) in Primary Airway Epithelial Cells of Asthmatic Mice.; Authors - Haipei Liu
mouse	IHC,IF	J Dermatol Sci; The temperature-sensitive receptors TRPV4 and TRPM8 have important roles in the pruritus of rosacea; Authors - Xiyuan Zhou
human,mouse	IHC	Cancer Gene Ther; TRPM8 promotes hepatocellular carcinoma progression by inducing SNORA55 mediated nuclear-mitochondrial communication; Authors - Jie Fu