TRMT6 Recombinant antibody

TRMT6 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240911D3

Cat no : 83843-6-RR

Synonyms

TRMT 6, TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, CGI-09, CGI 09

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Validation Data Gallery

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  WB analysis using 83843-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83843-6-RR (TRMT6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovarian cancer using 83843-6-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83843-6-RR (TRMT6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 83843-6-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRMT6 antibody (83843-6-RR, Clone: 240911D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83843-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83843-6-RR against Human TRMT6 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: U-251 cells, U-87 MG cells, HepG2 cells, SMMC-7721 cells, HuH-7 cells, mouse brain tissue
• Positive IHC detected in: human ovarian cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83843-6-RR is TRMT6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRMT6 fusion protein Ag10176
• Full Name: tRNA methyltransferase 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC001262
• Gene Symbol: TRMT6
• Gene ID (NCBI): 51605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR.