TRMT6 Recombinant antibody, PBS Only

TRMT6 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240911C5

Cat no : 83843-5-PBS

Synonyms

TRMT 6, TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, CGI-09, CGI 09

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Validation Data Gallery

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  WB analysis using 83843-5-RR (same clone as 83843-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83843-5-RR (TRMT6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83843-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 83843-5-RR (same clone as 83843-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83843-5-RR (TRMT6 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83843-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83843-5-RR (same clone as 83843-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRMT6 antibody (83843-5-RR, Clone: 240911C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83843-5-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83843-5-RR (same clone as 83843-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRMT6 antibody (83843-5-RR, Clone: 240911C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83843-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83843-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83843-5-RR against Human TRMT6 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83843-5-PBS is TRMT6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRMT6 fusion protein Ag10176
• Full Name: tRNA methyltransferase 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC001262
• Gene Symbol: TRMT6
• Gene ID (NCBI): 51605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA.