TRMT6 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRMT6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 16727-1-AP

Synonyms

CGI 09, GCD10, Gcd10p, KIAA1153, TRM6, TRMT6, tRNA(m1A58)MTase subunit TRM6

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Validation Data Gallery

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  WB analysis using 16727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16727-1-AP (TRMT6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16727-1-AP

  IP result of anti-TRMT6 (IP:16727-1-AP, 4ug; Detection:16727-1-AP 1:700) with HeLa cells lysate 2000 ug.

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  IHC staining of human colon cancer using 16727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16727-1-AP (TRMT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16727-1-AP (TRMT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HuH-7 cells, SMMC-7721 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

This antibody works well in ProteinSimple system.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

16727-1-AP targets TRMT6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRMT6 fusion protein Ag10176
• Full Name: tRNA methyltransferase 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC001262
• Gene Symbol: TRMT6
• Gene ID (NCBI): 51605
• RRID: AB_2878306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR. This antibody is specific to 56kd TRMT6 protein.

Publications

Species	Application	Title
mouse	WB	Acta Pharm Sin B; ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17; Authors - Junting Zhang
mouse	WB, IHC	Nat Commun; N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.; Authors - Yanying Wang
human	WB	Cell Discov; ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis.; Authors - Wenjun Kuang
mouse	WB	Nat Immunol; tRNA-m1A modification promotes T cell expansion via efficient MYC protein synthesis; Authors - Yongbo Liu ; KO Validated
human	WB	Cell Death Discov; N1-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction; Authors - Zhangyang Qi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Estefania (Verified Customer) (05-16-2022)

Very good signal. Tested using simple western (ProteinSimple)

• Applications: Western Blot
• Primary Antibody Dilution: 1:20
• Cell Tissue Type: HepG2 cell lysate

TRMT6 Antibody Western Blot validation (1:20 dilution) in HepG2 cell lysate (Cat no:16727-1-AP)