TRMT5 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRMT5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18255-1-AP

Synonyms

KIAA1393, M1G methyltransferase, TRM5, TRMT5, tRNA [GM37] methyltransferase, tRNA methyltransferase 5

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Validation Data Gallery

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  WB analysis of MCF-7 using 18255-1-AP

  WB result of TRMT5 antibody (18255-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT5 transfected MCF-7 cells.

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  WB analysis of Jurkat using 18255-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18255-1-AP (TRMT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 18255-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18255-1-AP (TRMT5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 18255-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18255-1-AP (TRMT5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse testis tissue, NIH/3T3 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

18255-1-AP targets TRMT5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRMT5 fusion protein Ag13075
• Full Name: TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae)
• Calculated Molecular Weight: 509 aa, 58 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC108284
• Gene Symbol: TRMT5
• Gene ID (NCBI): 57570
• RRID: AB_2209625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,rat	WB	Front Mol Biosci; Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy.; Authors - Huafeng Song
human,mouse	WB	J Zhejiang Univ Sci B; Targeting TRMT5 suppresses hepatocellular carcinoma progression via inhibiting the HIF-1α pathways; Authors - Qiong Zhao ; KD Validated