TRIP6 Monoclonal antibody

TRIP6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

5D8C7

Cat no : 60205-1-Ig

Synonyms

OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1

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Validation Data Gallery

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  WB analysis of HeLa using 60205-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60205-1-Ig (TRIP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60205-1-Ig (TRIP6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60205-1-Ig (TRIP6 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60205-1-Ig (TRIP6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 60205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60205-1-Ig(TRIP6 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Hela cells
• Positive IHC detected in: human ovary tumor tissue, human liver tissue, human colon cancer tissue, human colon tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

60205-1-Ig targets TRIP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIP6 fusion protein Ag15149
• Full Name: thyroid hormone receptor interactor 6
• Calculated Molecular Weight: 476 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC004249
• Gene Symbol: TRIP6
• Gene ID (NCBI): 7205
• RRID: AB_10973674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIP6 (thyroid receptor-interacting protein 6), also known as ZRP-1 (zyxin-related protein 1), is a member of the zyxin family that has been implicated in actin reorganization and cell motility. Trip6 has been proposed to transport signals from the cell surface to the nucleus.

Publications

Species	Application	Title
	WB	Dev Cell; Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex.; Authors - Marcela Raices