TRIP4 Polyclonal antibody, PBS Only

TRIP4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12324-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HL-60 using 12324-1-AP (same clone as 12324-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12324-1-AP (same clone as 12324-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12324-1-AP (same clone as 12324-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 12324-1-AP (same clone as 12324-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 12324-1-AP (same clone as 12324-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

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Product Information

12324-1-PBS targets TRIP4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIP4 fusion protein Ag2987
• Full Name: thyroid hormone receptor interactor 4
• Calculated Molecular Weight: 581 aa, 66 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC012448
• Gene Symbol: TRIP4
• Gene ID (NCBI): 9325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Thyroid hormone receptor interactor 4(TRIP4), other named ASC1, is a transcriptional coactivator that promote transcriptional efficiencies. It harbors an autonomous transactivation domain that contains a putative zinc finger motif, which serves as a binding site for nuclear receptors for thyroid, estrogen, all-trans-retinoic acid and RXR by interaction with SRC-1 or CBP/p300. There are two forms of ASC1 have been detected in mRNA level, isoform1(581aa, ~65kd) and isoform2(539aa,~60kd)(12077347,12390891)